Expression Optimization

  • Routine testing of multiple expression/induction conditions to optimize protein expression

  • Anaylsis by SDS-PAGE and Western Blot of crude, soluble and pull down fractions

Protein Purification

  • Pilot to bulk scale

  • Endogenous and recombinant protein production

  • Protein refolding from inclusion bodies

  • Biochemical and biophysical assay grade purity

  • Protease cleavage of fusion and purification tags

  • Affinity tag and enzymatic modifications for assay development

  • 15N, 13C, 2H (D) labeling for structural determination by NMR

Quality Control/ Analytics

  • SDS-PAGE

  • Analytical SEC

  • Static light scattering

  • Western blot

  • Activity Assays

  • Mass spectrometry