Expression Optimization

  • Routine testing of multiple expression/induction conditions to optimize protein expression
  • Anaylsis by SDS-PAGE and Western Blot of crude, soluble and pull down fractions

Protein Purification

  • Pilot to bulk scale
  • Endogenous and recombinant protein production
  • Protein refolding from inclusion bodies
  • Biochemical and biophysical assay grade purity
  • Protease cleavage of fusion and purification tags
  • Affinity tag and enzymatic modifications for assay development
  • 15N, 13C labeling for structural determination by NMR

Quality Control/ Analytics

  • SDS-PAGE
  • Analytical SEC
  • Static light scattering
  • Western blot
  • Activity Assays
  • Mass spectrometry